Initiation of primary cell cultures from embryonic Haemaphysalis bispinosa ticks

• DOI: 10.11158/saa.22.3.1
• Publication Date: 2017-02-09T19:17:16Z
• Authors: Fang-shiang Lim; Jing-jing Khoo; Fezshin Chen; Lesley Bell-sakyi; Chee-sieng Khor; Li-yen Chang; Sazaly Abu Bakar
• Keywords: Haemaphysalis

Tick cell cultures have been widely used as an important tool for the study of tick-associated microorganisms, specifically medically bacteria or viruses that may be difficult to isolate culture in axenic conditions. In this study, primary embryonic tick were initiated separately from each egg batches laid by 10 female ticks belonging hard genus Haemaphysalis. All maintained at 28°C. After months, 4 healthy identified with potential developing into continuous lines. These comprise large cells predominantly forming floating clumps multicellular vesicles, which are morphologically similar lines derived soft Ornithodoros moubata. Subculture has not yet performed due low density time writing. Amplification and sequencing a fragment 16S rRNA gene DNA extracted parent showed 99%-100% similarity published sequences Haemaphysalis bispinosa. This is first report initiation found Malaysia. Such will useful studies tick-borne pathogens region, where recent shown highly represented harbor bacteria.