The Effect of Sterilization on the Bovine Pericardium Scaffold Decellularized By the Glutaraldehyde-Free Technology

• DOI: 10.11159/jbeb.2021.004
• Publication Date: 2021-12-24T14:54:02Z
• Authors: Nataliia V. Shcho...; Anatoliy A. Sokol; Liudmyla V. Dolin...; Oleksandr Yu. Galkin; Glib I. Glib I.; Dmytro A. Yemets; Anatoliy A. Sokol; Arkadii A. Dovgha...; Iryna M. Skorokhod; Olena V. Olena V.; Nadiia M. Rudenko; Iliia M. Yemets
• Keywords: Decellularization; Glutaraldehyde; Sterilization

The use of xenotissue for the needs regenerative and cardiovasculare medicine is a promising area tissue engineering.The decellularization process provides complete purification elastin-collagen matrix bovine pericardium from cells their components.The high concentrations sodium dodecyl sulfate glutaraldehyde can lead to damage extracellular matrix.Therefore, purpose this study was microarchitectonics decellularized using low concentration (0.1% solution) avoiding glutaraldehyde.Further stabilization fixation carried out 10 mM 1- carbodiimide hydrochloride N-Hydroxysuccinimide. effect assessed by staining samples with hematoxylin-eosin scanning electron microscopy.Also research results confirmed absence structural changes in collagenelastin fibers after sterilization dose kGy.Thus, it be assumed that radiation method may safe bioimplants.