Optical microscopy and spectroscopy of analyte-sensitive functionalized gold nanoparticles in microfluidic systems

• DOI: 10.1117/12.2001608
• Publication Date: 2013-03-14T21:54:00Z
• Authors: Martinus H. V. Werts; Vincent Raimbault; Matthieu Loumaigne; Laurent Griscom; Olivier Français; Julien R. G. Navarro; Anne Débarre; Bruno Le Pioufle
• Keywords: Gold Colloid

The use of microfluidic devices for the handling and analysis suspensions colloidal gold particles is presented. plasmonic are detected <i>via</i> their resonant light scattering (RLS) in a simple versatile LED-based dark field illumination geometry. RLS enables both microscopic imaging microspectroscopy. measurement diffusion coefficient nanoparticles this type demonstrated. Nanoparticles separated from small (free ligand) molecules continuous flow process. Reversible aggregation functionalized microflow by means RLS, which opens perspectives development bioplasmonic detection schemes.