<title>Determination of multiple analytes using a fiber optic biosensor based on fluorescence energy transfer</title>

• DOI: 10.1117/12.237624
• Publication Date: 2004-10-12T22:32:01Z
• Authors: Richard B. Thompson; Zhengfang Ge; Marcia W. Patchan; Carol A. Fierke; Keith A. McCall; Daniel Elbaum; David W. Christia...
• Keywords: Cuvette

Recently, we have developed a biosensor for zinc based on the very tight binding of this metal by enzyme carbonic anhydrase, which requires Zn(II) catalysis. We were able to transduce as change in fluorescence intensity or lifetime use colored inhibitor whose metal-dependent permits resonance energy transfer (Forster transfer) occur. extended concept include other metals and analytes may be bound native (or mutant) active site with concomitant color change; is transduced efficiency. also recently demonstrated similar approach, wherein presence ion anisotropy. Results cuvettes fiber optic sensors are shown.