Pixel-level analysis of protein markers in highly multiplexed immunofluorescence images of kidney biopsies

• DOI: 10.1117/12.3008522
• Publication Date: 2024-04-02T20:35:20Z
• Authors: Madeleine S. Durkee; Junting Ai; Gabriel Casella; Thao Cao; Michael S. Andrade; Marcus R. Clark; Maryellen L. Giger
• Keywords: Immunofluorescence

The goal of this work is to reduce the complexity cell and neighborhood annotations in studies for spatial immunity. Specifically, we use a method inspired by spectral angle mapping collapse multichannel images into class-level representations. We will demonstrate that these class maps assist characterizing immune infiltration renal pathologies.