Effect of Aerobic/Strength Training on RANKL Gene DNA Methylation Levels

• DOI: 10.1123/jpah.2022-0245
• Publication Date: 2023-06-09T07:43:34Z
• Authors: Ameni Chelly; Amal Bouzid; Fadoua Neifar; Ines Kammoun; Adel Tekari; Saber Masmoudi; Hamdi Chtourou; Ahmed Rebai
• Keywords: Aerobic Exercise; CpG site; Bisulfite sequencing

The osteoclastogenesis RANKL gene plays a key role in bone remodeling. hypomethylation of its promoter region may cause osteoporosis. present study aimed to elucidate the influence physical activity on DNA methylation changes cytosine-phosphate-guanine (CpG)-rich active and sedentary adults assess effect aerobic strength training among Tunisian-North African adults.A total 104 participants including 52 (58% males 42% females) (31% 69% were recruited for observational interventional part study, respectively. intervention consisted 12 weeks (30 min/session) followed by 10 minutes strengthening exercises. All completed International Physical Activity Questionnaire provided blood samples quantitative methylation-specific polymerase chain reaction (PCR) analysis.The revealed significant difference (P = 6 × 10-10) level between adults, with 6.68-fold increase observed group. After intervention, both trained 41 10-5) untrained .002) groups displayed high levels region. In addition, group exhibited improvements heart rate 2.2 10-16), pressure 39 10-3), maximal oxygen uptake 1.5 10-7), fat mass 7 10-4).Exploring epigenetic modifications contribute more comprehensive understanding complexity This suggests that aerobic/strength could potentially improve system, reducing vulnerability osteoporosis increasing methylation.