The Role of ATP-Sensitive Potassium Channels in Neutrophil Migration and Plasma Exudation

• DOI: 10.1124/jpet.300.3.946
• Publication Date: 2003-01-10T18:32:29Z
• Authors: José Eduardo Da S...; Maria Cláudia San...; Fernando de Queir...; Jamil Assreuy
• Keywords: Potassium channel blocker; ATP-sensitive potassium channel; Channel blocker; Leukotriene B4; Cromakalim

Neutrophil activation and migration during an inflammatory response is preceded or accompanied by plasma membrane electrical changes. Besides changes in calcium currents, neutrophils have a high permeability to potassium, mainly through potassium channels. However, the significance of channels neutrophil physiology still unclear. Here, we show that treatment rats with ATP-sensitive channel blocker glibenclamide (4, 20, 40 μmol/kg) dose dependently decreased carrageenan-,<i>N</i>-formyl-methionyl-leucyl-phenylalanine (fMLP)-, lipopolysaccharide-induced influx fluid leakage into interpleural space. On other hand, minoxidil (an opener; 25, 50, 100 increased both induced submaximal carrageenan. In addition, vitro human chemotaxis leukotriene B₄ fMLP (both 1 μM) was fully blocked (10, 30, tetraethylammonium (a nonselective blocker; 1, 3, 10 mM). Thus, our results disclose possibility may role exudation response.