Enhancement of Glucose Transporter Expression of Brain Endothelial Cells by Vascular Endothelial Growth Factor Derived from Glioma Exposed to Hypoxia

• DOI: 10.1124/mol.107.038851
• Publication Date: 2007-10-18T01:39:07Z
• Authors: Wei-Lan Yeh; Chun-Jung Lin; Wen-Mei Fu
• Keywords: Hypoxia

Increased need for glycolysis and glucose uptake ATP production is observed in tumor cells, particularly cells lacking of oxygen supply. Because transported from blood to tumor, molecules must be delivered across transporters the vascular endothelium cells. Here we found that glioma suffered hypoxic insults can secrete factor(s) regulate transporter expression brain endothelium. It was conditioned medium rat C6 under hypoxia up-regulated type 1 (GLUT1) endothelial whereas normoxia caused no significant effect. We further investigated whether potentiating effect by growth factor (VEGF) because secreted VEGF markedly increased condition. By transfection with small interfering RNA, it transfected longer GLUT1 Moreover, addition VEGF-neutralizing antibody could also exert similar inhibitory effects. Furthermore, VEGF-induced increase mediated phosphoinositide-3 kinase/Akt pathway. Our results indicate may transport blood-brain barrier.