Functional Interaction between Trace Amine-Associated Receptor 1 and Dopamine D2 Receptor

• DOI: 10.1124/mol.111.073304
• Publication Date: 2011-06-14T03:00:27Z
• Authors: Stefano Espinoza; Ali Salahpour; Bernard Masri; Tatyana D. Sotnikova; Mirko Messa; Larry S. Barak; Marc G. Caron; Raul R. Gainetdinov
• Keywords: TRACE (psycholinguistics)

The ability of dopamine receptors to interact with other receptor subtypes may provide mechanisms for modulating dopamine-related functions and behaviors. In particular, there is evidence suggesting that the trace amine-associated 1 (TAAR1) affects dopaminergic system by regulating firing rate neurons or altering D2 (D2R) responsiveness ligands. TAAR1 a Gα_s protein-coupled activated biogenic amines, "trace amines," such as β-phenylethylamine (β-PEA) tyramine are normally found at low concentrations in mammalian brain. present study, we investigated biochemical mechanism interaction between D2R role this plays D2R-related signaling Using bioluminescence resonance energy transfer biosensor cAMP, demonstrated antagonists haloperidol, raclopride, amisulpride were able enhance selectively TAAR1-mediated β-PEA increase cAMP. Moreover, form heterodimers when coexpressed human embryonic kidney 293 cells, direct was disrupted presence haloperidol. addition, mice lacking TAAR1, haloperidol-induced striatal c-Fos expression catalepsy significantly reduced. Taken together, these data suggest have functional physical interactions could be critical modulation vivo.