Transient Receptor Potential Vanilloid-1 (TRPV1) Is a Mediator of Lung Toxicity for Coal Fly Ash Particulate Material

• DOI: 10.1124/mol.111.076067
• Publication Date: 2011-12-10T02:57:31Z
• Authors: Cassandra E. Deer...; Mark E. Johansen; Jessica K. Roberts; Karen C. Thomas; Erin G. Romero; Jeewoo Lee; Garold S. Yost; John M. Veranth; Christopher A. Re...
• Keywords: Mediator

Environmental particulate matter (PM) pollutants adversely affect human health, but the molecular basis is poorly understood. The ion channel transient receptor potential vanilloid-1 (TRPV1) has been implicated as a sensor for environmental PM and mediator of adverse events in respiratory tract. objectives this study were to determine whether TRPV1 can distinguish chemically physically unique that represents important sources air pollution; elucidate activation by PM; ascertain contributions lung cell mouse tissue responses exposed an insoluble agonist, coal fly ash (CFA1). major findings are activated some, not all prototype materials evaluated, with rank-ordered CFA1 > diesel exhaust crystalline silica; TRP melastatin-8 also robustly CFA1, whereas other channels expressed airway sensory neurons epithelial cells may be including TRPs ankyrin 1 (A1), canonical 4α (C4α), M2, V2, V3, V4, either slightly (TRPA1) or CFA1; occurs via surface interactions between solid components specific amino acid residues localized putative pore-loop region; exclusive lungs pathway which affects expression selected genes tissue.