Regions on Adenylyl Cyclase VII Required for Selective Regulation by the G13 Pathway

• DOI: 10.1124/mol.112.082446
• Publication Date: 2012-12-11T04:03:28Z
• Authors: Lily I. Jiang; Jennifer E. Wang; Paul C. Sternweis
• Keywords: ADCY10

Regulation of multiple adenylyl cyclases (AC) provides unique inputs to mediate the synthesis cAMP, a ubiquitous second messenger that controls many aspects cellular function. On stimulation by G_s, activities ACs can be further selectively modulated other pathways ensure precise control intracellular cAMP responses specific stimuli. Recently, we reported one AC isoforms, AC7, is uniquely regulated G₁₃ pathway. To understand more fully molecular mechanism this regulation, compared regulation AC7 with AC2 in bone marrow–derived macrophages devoid AC7. Although both enzymes could restore G<i>βγ</i>, activation pathway preferentially synergized Exchange domains between two isoforms indicates C1b domain and N-terminus C1a are important for directing selective A mutagenesis screen identified regions differentially its distinct pathways.