Disabling Cas9 by an anti-CRISPR DNA mimic

• DOI: 10.1126/sciadv.1701620
• Publication Date: 2017-07-13T01:00:34Z
• Authors: Jiyung Shin; Fuguo Jiang; Jun-Jie Liu; Nicolas L. Bray; Benjamin J. Rauch; Seung Hyun Baik; Eva Nogales; Joseph Bondy-Denomy; Jacob E. Corn; Jennifer A. Doudna
• Keywords: Models, Molecular; 0301 basic medicine; 570; 1.1 Normal biological development and functioning; Molecular Conformation; RNA, Guide, CRISPR-Cas Systems; Cell Line; Structure-Activity Relationship; 03 medical and health sciences; Models; CRISPR-Associated Protein 9; Genetics; 2.1 Biological and endogenous factors; Humans; Clustered Regularly Interspaced Short Palindromic Repeats; Gene Silencing; Research Articles; Gene Editing; Molecular; High-Throughput Nucleotide Sequencing; DNA; Biological Sciences; 3. Good health; 5.1 Pharmaceuticals; RNA; Biochemistry and Cell Biology; Generic health relevance; CRISPR-Cas Systems; Guide; Biotechnology

Natural inhibitors of Cas9 pretend to be DNA and block target binding, and using them in human cells can reduce off-target events.