Biophysical basis underlying dynamic Lck activation visualized by ZapLck FRET biosensor

• DOI: 10.1126/sciadv.aau2001
• Publication Date: 2019-06-19T23:14:08Z
• Authors: Rongxue Wan; Jenny Wu; Mingxing Ouyang; Lei Lei; Jiaming Wei; Qin Peng; Reed Harrison; Yiqian Wu; Binbin Cheng; Kaitao Li; Cheng Zhu; Liling Tang; Yingxiao Wang; Shaoying Lu
• Keywords: 0303 health sciences; Receptors, Antigen, T-Cell; Biosensing Techniques; Cell Line; Jurkat Cells; 03 medical and health sciences; HEK293 Cells; src-Family Kinases; Lymphocyte Specific Protein Tyrosine Kinase p56(lck); Cell Line, Tumor; Fluorescence Resonance Energy Transfer; Humans; Phosphorylation; Research Articles; HeLa Cells; Signal Transduction

A new biosensor was engineered to visualize Lck kinase preactivation and regulation in live T cells.