Decellularized extracellular matrix scaffolds identify full-length collagen VI as a driver of breast cancer cell invasion in obesity and metastasis

Decellularization
DOI: 10.1126/sciadv.abc3175 Publication Date: 2020-10-21T23:18:17Z
ABSTRACT
The extracellular matrix (ECM), a major component of the tumor microenvironment, promotes local invasion to drive metastasis. Here, we describe method study whole-tissue ECM effects from disease states associated with metastasis on cell phenotypes and identify individual proteins signaling pathways that are driving these effects. We show decellularized tumor-bearing obese mammary glands drives TNBC invasion. Proteomics gland led us full-length collagen VI as novel driver whose abundance in stroma increases body mass index human patients. Last, mechanism by which contributes via NG2-EGFR cross-talk MAPK signaling. Overall, studies demonstrate value scaffolds obtained tissues functions ECM.
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