Decellularized extracellular matrix scaffolds identify full-length collagen VI as a driver of breast cancer cell invasion in obesity and metastasis

• DOI: 10.1126/sciadv.abc3175
• Publication Date: 2020-10-21T23:18:17Z
• Authors: Andrew L. Wishart; Sydney J. Conner; Justinne R. Guarin; Jackson P. Fatherree; Yifan Peng; Rachel A. McGinn; Rebecca Crews; Stephen P. Naber; Martin Hunter; Andrew S. Greenberg; Madeleine J. Oudin
• Keywords: Decellularization

The extracellular matrix (ECM), a major component of the tumor microenvironment, promotes local invasion to drive metastasis. Here, we describe method study whole-tissue ECM effects from disease states associated with metastasis on cell phenotypes and identify individual proteins signaling pathways that are driving these effects. We show decellularized tumor-bearing obese mammary glands drives TNBC invasion. Proteomics gland led us full-length collagen VI as novel driver whose abundance in stroma increases body mass index human patients. Last, mechanism by which contributes via NG2-EGFR cross-talk MAPK signaling. Overall, studies demonstrate value scaffolds obtained tissues functions ECM.