Targeting oncoproteins with a positive selection assay for protein degraders

• DOI: 10.1126/sciadv.abd6263
• Publication Date: 2021-02-08T23:22:34Z
• Authors: Vidyasagar Koduri; Leslie Duplaquet; Benjamin L. Lampson; Adam C. Wang; Amin H. Sabet; Mette Ishoey; Joshiawa Paulk; Mingxing Teng; Isaac S. Harris; Jennifer E. Endress; Xiaoxi Liu; Ethan Dasilva; Joao A. Paulo; Kimberly J. Briggs; John G. Doench; Christopher J. Ott; Tinghu Zhang; Katherine A. Donovan; Eric S. Fischer; Steven P. Gygi; Nathanael S. Gray; James Bradner; Jeffrey A. Medin; Sara J. Buhrlage; Matthew G. Oser; William G. Kaelin
• Keywords: Oncogene Proteins; 0301 basic medicine; Benzylamines; Thalidomide; Ikaros Transcription Factor; 03 medical and health sciences; Proteolysis; Basic Helix-Loop-Helix Transcription Factors; Quinazolines; Humans; CRISPR-Cas Systems; Research Articles; Adaptor Proteins, Signal Transducing; Transcription Factors

A new positive selection assay could enhance the discovery of compounds that degrade previously undruggable proteins.