Intermediate cells of in vitro cellular reprogramming and in vivo tissue regeneration require desmoplakin

• DOI: 10.1126/sciadv.abk1239
• Publication Date: 2022-10-28T17:58:35Z
• Authors: Jeongmin Ha; Bum Suk Kim; Byungkuk Min; Juhyeon Nam; Jae-Geun Lee; Minhyung Lee; Byoung-Ha Yoon; Yoon Ha Choi; Ilkyun Im; Jung Sun Park; Hyosun Choi; Areum Baek; Sang Mi Cho; Mi-Ok Lee; Ki-Hoan Nam; Ji Young Mun; Mirang Kim; Seon-Young Kim; Mi Young Son; Yong-Kook Kang; Jeong-Soo Lee; Jong Kyoung Kim; Janghwan Kim
• Keywords: Reprogramming; Desmoplakin

Amphibians and fish show considerable regeneration potential via dedifferentiation of somatic cells into blastemal cells. In terms dedifferentiation, in vitro cellular reprogramming has been proposed to share common processes with vivo tissue regeneration, although the details are elusive. Here, we identified cytoskeletal linker protein desmoplakin (Dsp) as a factor mediating both regeneration. Our analysis revealed that Dsp expression is elevated distinct intermediate during reprogramming. Knockdown impedes induced pluripotent stem neural stem/progenitor well zebrafish fins. Notably, reduced impairs formation These findings suggest there Dsp-mediated evolutionary link between mammals lower vertebrates may provide alternative approaches for mammalian regenerative therapy.