Depletion of oocyte dynamin-related protein 1 shows maternal-effect abnormalities in embryonic development

• DOI: 10.1126/sciadv.abl8070
• Publication Date: 2022-06-15T17:53:55Z
• Authors: Deepak Adhikari; In-won Lee; Usama Al-Zubaidi; Jun Liu; Qing-Hua Zhang; Wai Shan Yuen; Likun He; Yasmyn Winstanley; Hiromi Sesaki; Jeffrey R. Mann; Rebecca L. Robker; John Carroll
• Keywords: Oocyte activation; Epigenome; DNM1L

Eggs contain about 200,000 mitochondria that generate adenosine triphosphate and metabolites essential for oocyte development. Mitochondria also integrate metabolism transcription via regulate epigenetic modifiers, but there is no direct evidence linking mitochondrial function to the maternal epigenome subsequent embryo Here, we have disrupted deletion of fission factor Drp1. Fission-deficient oocytes exhibit a high frequency failure in peri- postimplantation This associated with altered function, changes transcriptome proteome, subcortical complex, decrease DNA methylation H3K27me3. Transplanting pronuclei fertilized Drp1 knockout normal ooplasm fails rescue embryonic lethality. We conclude plays role establishing epigenome, serious consequences