Coordinated regulation of RNA polymerase II pausing and elongation progression by PAF1

• DOI: 10.1126/sciadv.abm5504
• Publication Date: 2022-04-01T17:59:57Z
• Authors: Zhenning Wang; Aixia Song; Hao Xu; Shibin Hu; Bolin Tao; Linna Peng; Jingwen Wang; Jiabei Li; Jiali Yu; Li Wang; Ze Li; Xizi Chen; Mengyun Wang; Yayun Chi; Jiong Wu; Yanhui Xu; Hai Zheng; Fei Xavier Chen
• Keywords: RNA polymerase II; Transcription; RNA polymerase III

Pleiotropic transcription regulator RNA polymerase II (Pol II)–associated factor 1 (PAF1) governs multiple transcriptional steps and the deposition of several epigenetic marks. However, it remains unclear how ultimate outcome is determined by PAF1 whether relates to PAF1-controlled We use rapid degradation systems reveal direct functions in governing pausing partially recruiting Integrator-PP2A (INTAC), addition ensuring elongation. Following acute degradation, most destabilized undergoes effective release, which presumably relies on skewed balance between INTAC P-TEFb, resulting hyperphosphorylated substrates including SPT5. Impaired Pol progression during elongation, along with altered pause release frequency, determines final outputs. Moreover, causes a cumulative decline histone modifications. These alterations chromatin likely further influence production transcripts from target genes.