Coordinated regulation of RNA polymerase II pausing and elongation progression by PAF1

RNA polymerase II Transcription RNA polymerase III
DOI: 10.1126/sciadv.abm5504 Publication Date: 2022-04-01T17:59:57Z
ABSTRACT
Pleiotropic transcription regulator RNA polymerase II (Pol II)–associated factor 1 (PAF1) governs multiple transcriptional steps and the deposition of several epigenetic marks. However, it remains unclear how ultimate outcome is determined by PAF1 whether relates to PAF1-controlled We use rapid degradation systems reveal direct functions in governing pausing partially recruiting Integrator-PP2A (INTAC), addition ensuring elongation. Following acute degradation, most destabilized undergoes effective release, which presumably relies on skewed balance between INTAC P-TEFb, resulting hyperphosphorylated substrates including SPT5. Impaired Pol progression during elongation, along with altered pause release frequency, determines final outputs. Moreover, causes a cumulative decline histone modifications. These alterations chromatin likely further influence production transcripts from target genes.
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