Multiplexed high-throughput immune cell imaging reveals molecular health-associated phenotypes

• DOI: 10.1126/sciadv.abn5631
• Publication Date: 2022-11-02T17:57:27Z
• Authors: Yannik Severin; Benjamin D. Hale; Julien Mena; David Goslings; Beat M. Frey; Berend Snijder
• Keywords: Inflammation; 0301 basic medicine; 0303 health sciences; 03 medical and health sciences; Phenotype; Humans; Biomedicine and Life Sciences; Neural Networks, Computer; 3. Good health

Phenotypic plasticity is essential to the immune system, yet the factors that shape it are not fully understood. Here, we comprehensively analyze immune cell phenotypes including morphology across human cohorts by single-round multiplexed immunofluorescence, automated microscopy, and deep learning. Using the uncertainty of convolutional neural networks to cluster the phenotypes of eight distinct immune cell subsets, we find that the resulting maps are influenced by donor age, gender, and blood pressure, revealing distinct polarization and activation-associated phenotypes across immune cell classes. We further associate T cell morphology to transcriptional state based on their joint donor variability and validate an inflammation-associated polarized T cell morphology and an age-associated loss of mitochondria in CD4 + T cells. Together, we show that immune cell phenotypes reflect both molecular and personal health information, opening new perspectives into the deep immune phenotyping of individual people in health and disease.