1000 spider silkomes: Linking sequences to silk physical properties

• DOI: 10.1126/sciadv.abo6043
• Publication Date: 2022-10-12T17:57:16Z
• Authors: Kazuharu Arakawa; Nobuaki Kono; Ali D. Malay; Ayaka Tateishi; Nao Ifuku; Hiroyasu Masunaga; Ryota Sato; Kousuke Tsuchiya; Rintaro Ohtoshi; Daniel Pedrazzoli; Asaka Shinohara; Yusuke Ito; Hiroyuki Nakamura; Akio Tanikawa; Yuya Suzuki; Takeaki Ichikawa; Shohei Fujita; Masayuki Fujiwara; Masaru Tomita; Sean J. Blamires; Jo-Ann Chuah; Hamish Craig; Choon P. Foong; Gabriele Greco; Juan Guan; Chris Holland; David L. Kaplan; Kumar Sudesh; Biman B. Mandal; Y. Norma-Rashid; Nur A. Oktaviani; Rucsanda C. Preda; Nicola M. Pugno; Rangam Rajkhowa; Xiaoqin Wang; Kenjiro Yazawa; Zhaozhu Zheng; Keiji Numata
• Keywords: Spider Silk; Biomaterial

Spider silks are among the toughest known materials and thus provide models for renewable, biodegradable, sustainable biopolymers. However, entirety of their diversity still remains elusive, that exceed performance limits industrial fibers constantly being found. We obtained transcriptome assemblies from 1098 species spiders to comprehensively catalog silk gene sequences measured mechanical, thermal, structural, hydration properties dragline 446 species. The combination these protein genotype-phenotype data revealed essential contributions multicomponent structures with major ampullate spidroin 1 3 paralogs in high-performance numerous amino acid motifs contributing each properties. hope our global sampling, comprehensive testing, integrated analysis, open will a solid starting point future biomaterial designs.