Shell microelectrode arrays (MEAs) for brain organoids

• DOI: 10.1126/sciadv.abq5031
• Publication Date: 2022-08-17T17:57:37Z
• Authors: Qi Huang; Bohao Tang; July Carolina Romero; Yuqian Yang; Saifeldeen Khalil...; Gayatri Pahapale; Tien-Jung Lee; Itzy E. Morales P...; Fang Han; Cynthia Berlinicke; Terry Xiang; Mallory Solazzo; Thomas Hartung; Zhao Qin; Brian S. Caffo; Lena Smirnova; David H. Gracias
• Keywords: Organoid; Multielectrode array

Brain organoids are important models for mimicking some three-dimensional (3D) cytoarchitectural and functional aspects of the brain. Multielectrode arrays (MEAs) that enable recording stimulation activity from electrogenic cells offer notable potential interrogating brain organoids. However, conventional MEAs, initially designed monolayer cultures, limited contact area restricted to bottom 3D Inspired by shape electroencephalography caps, we developed miniaturized wafer-integrated MEA caps The optically transparent shells composed self-folding polymer leaflets with conductive polymer–coated metal electrodes. Tunable folding minicaps’ guided mechanics simulations enables versatile different sizes, validate feasibility electrophysiology 400- 600-μm-sized up 4 weeks in response glutamate stimulation. Our studies suggest shell MEAs great high signal-to-noise ratio spatiotemporal organoid recording.