A single-cell multi-omic atlas spanning the adult rhesus macaque brain

• DOI: 10.1126/sciadv.adh1914
• Publication Date: 2023-10-12T17:57:22Z
• Authors: Kenneth L. Chiou; Xingfan Huang; Martin O. Bohlen; Sébastien Tremblay; Alex R. DeCasien; Diana R. O’Day; Cailyn H. Spurrell; Aishwarya A. Gogate; Trisha M. Zintel; ; Madeline G. Andrews; Melween I. Martínez; Lea M. Starita; Michael J. Montague; Michael L. Platt; Jay Shendure; Noah Snyder-Mackler
• Keywords: Rhesus macaque; Brain atlas; Cell type; Human brain

Cataloging the diverse cellular architecture of primate brain is crucial for understanding cognition, behavior, and disease in humans. Here, we generated a brain-wide single-cell multimodal molecular atlas rhesus macaque brain. Together, profiled 2.58 M transcriptomes 1.59 epigenomes from single nuclei sampled 30 regions across adult Cell composition differed extensively brain, revealing signatures region-specific functions. We also identified 1.19 candidate regulatory elements, many previously unidentified, allowing us to explore landscape cis-regulatory grammar neurological risk cell type–specific manner. Altogether, this multi-omic provides an open resource investigating evolution human identifying novel targets interventions.