Chromatin analysis of adult pluripotent stem cells reveals a unique stemness maintenance strategy

• DOI: 10.1126/sciadv.adh4887
• Publication Date: 2023-10-06T17:58:44Z
• Authors: Axel Poulet; Arcadia J. Kratki...; Danyan Li; Josien C. van Wol...
• Keywords: Pluripotent Stem Cells; Adult Stem Cells; Animals; Biomedicine and Life Sciences; Planarians; Promoter Regions, Genetic; Chromatin

Many highly regenerative organisms maintain adult pluripotent stem cells throughout their life, but how the long-term maintenance of pluripotency is accomplished unclear. To decipher regulatory logic cells, we analyzed chromatin organization cell genes in planarian Schmidtea mediterranea . We identify a special state genes, which distinct from that tissue-specific and resembles constitutive genes. Where promoters have detectable transcription factor binding sites, cell–specific instead sequence features broadly decrease nucleosome affinity. This genic makes pluripotency-related gene expression default these maintained by activity remodelers ISWI SNF2 cells.