Patient-derived exosomes facilitate therapeutic targeting of oncogenic MET in advanced gastric cancer

• DOI: 10.1126/sciadv.adk1098
• Publication Date: 2023-11-24T18:58:15Z
• Authors: Sujin Hyung; Jihoon Ko; You Jeong Heo; Steven M. Blum; Seung Tae Kim; Se Hoon Park; Joon Oh Park; Won Ki Kang; Ho Yeong Lim; Samuel J. Klempner; Jeeyun Lee
• Keywords: MicroRNAs; Stomach Neoplasms; Cell Line, Tumor; Humans; Ascites; Biomedicine and Life Sciences; Exosomes; 3. Good health

Gastric cancer (GC) with peritoneal metastases and malignant ascites continues to have poor prognosis. Exosomes mediate intercellular communication during progression promote therapeutic resistance. Here, we report the significance of exosomes derived from (EXO Ascites ) in use modified as resources for therapy. EXO patients GC stimulated invasiveness angiogenesis an ex vivo three-dimensional autologous tumor spheroid microfluidic system. concentration increased invasiveness, blockade their secretion suppressed progression. In MET -amplified GC, contain abundant MET; selective delivery cells enhanced invasiveness. Exosomal depletion substantially reduced invasiveness; additive effect was induced when combined and/or VEGFR2 inhibition a patient-derived model. Allogeneic MET-harboring exosome invasion non-amplified patient tissues showed higher than adjacent normal tissues. Manipulating content production may be promising complementary strategy against GC.