Development of an antigen-based approach to noninvasively image CAR T cells in real time and as a predictive tool

• DOI: 10.1126/sciadv.adn3816
• Publication Date: 2024-09-18T17:59:11Z
• Authors: Julia Fröse; Jennifer Rowley; Ali Salehi Farid; Taha Rakhshandehroo; Paul Leclerc; Howard Mak; Harris Allen; Heydar Moravej; Leila Munaretto; Luis Millan-Barea; Elisabeth Codet; Hannah Glockner; Caron Jacobson; Michael Hemann; Mohammad Rashidian
• Keywords: Homing (biology); Limiting

CAR T cell therapy has revolutionized the treatment of a spectrum blood-related malignancies. However, responses vary among cancer types and patients. Accurate monitoring dynamics is crucial for understanding evaluating efficacy. Positron emission tomography (PET) offers comprehensive view homing, especially in critical organs such as lymphoid structures bone marrow. This information will help assess response predict relapse risk. Current PET imaging methods require genetic modifications, limiting clinical use. To overcome this, we developed an antigen-based approach enabling whole-body imaging. The probe detects cells vivo without affecting their function. In immunocompetent B leukemia model, CAR-PET signal spleen predicted early mortality allows assessment altering established protocols. It seamlessly integrates with FDA-approved future generations, facilitating broader application.