In situ implantable DNA hydrogel for diagnosis and therapy of postoperative rehemorrhage following intracerebral hemorrhage surgery

• DOI: 10.1126/sciadv.ado3919
• Publication Date: 2024-08-14T18:00:39Z
• Authors: Wenyan Yu; Enpeng Gong; Changlin Wang; Chengyuan Che; Yuzhen Zhao; Xinyun Wu; Yi Yang; Haiyu Shi; Mengjuan Chen; Mingge Li; Li Xie; Yue Guo; Mingming Guo; Liya Mu; Zhenya Wang; Zhenzhong Zhang; Kaixiang Zhang; Junjie Liu; Jinjin Shi
• Keywords: Male; 0301 basic medicine; Iron; Hydrogels; DNA; Deferoxamine; Postoperative Hemorrhage; Aptamers, Nucleotide; Rats; Hemoglobins; Disease Models, Animal; 03 medical and health sciences; Animals; Humans; Ferroptosis; Biomedicine and Life Sciences; Cerebral Hemorrhage

Postoperative rehemorrhage following intracerebral hemorrhage surgery is intricately associated with a high mortality rate, yet there now no effective clinical treatment. In this study, we developed hemoglobin (Hb)-responsive in situ implantable DNA hydrogel comprising Hb aptamers cross-linked two complementary chains and encapsulating deferoxamine mesylate (DFO). Functionally, the generates signals upon postoperative by capturing Hb, demonstrating distinctive "self-diagnosis" capability. addition, ongoing capture of mediates gradual disintegration hydrogel, enabling on-demand release DFO without compromising physiological iron-dependent functions. This process achieves self-treatment inhibiting ferroptosis neurocytes. collagenase autologous blood injection model-induced mimic model, exhibited 5.58-fold increase iron absorption efficiency, reducing hematoma size significantly (from 8.674 to 4.768 cubic millimeters). innovative Hb-responsive not only offers therapeutic intervention for but also provides self-diagnosis feedback, holding notable promise enhancing outcomes.