CDCA7 is an evolutionarily conserved hemimethylated DNA sensor in eukaryotes
DNA (Cytosine-5-)-Methyltransferase 1
Adenosine Triphosphatases
0301 basic medicine
Ubiquitin-Protein Ligases
Cryoelectron Microscopy
Ubiquitination
DNA Helicases
Nuclear Proteins
Eukaryota
Cell Cycle Proteins
Zinc Fingers
DNA
DNA Methylation
Chromatin
Nucleosomes
Evolution, Molecular
Histones
03 medical and health sciences
CCAAT-Enhancer-Binding Proteins
Humans
CpG Islands
Biomedicine and Life Sciences
Protein Binding
DOI:
10.1126/sciadv.adp5753
Publication Date:
2024-08-23T17:58:55Z
AUTHORS (15)
ABSTRACT
Mutations of the SNF2 family ATPase HELLS and its activator CDCA7 cause immunodeficiency, centromeric instability, facial anomalies syndrome, characterized by DNA hypomethylation at heterochromatin. It remains unclear why CDCA7-HELLS is sole nucleosome remodeling complex whose deficiency abrogates maintenance methylation. We here identify unique zinc-finger domain as an evolutionarily conserved hemimethylation-sensing zinc finger (HMZF) domain. Cryo–electron microscopy structural analysis CDCA7-nucleosome reveals that HMZF can recognize hemimethylated CpG in outward-facing major groove within core particle, whereas UHRF1, critical methyltransferase DNMT1, cannot. recruits to chromatin facilitates UHRF1-mediated H3 ubiquitylation associated with replication-uncoupled propose assists methylation on sensing otherwise inaccessible UHRF1 DNMT1.
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CITATIONS (3)
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