Scalable acoustic virtual stirrer for enhanced interfacial enzymatic nucleic acid reactions

• DOI: 10.1126/sciadv.adt6955
• Publication Date: 2025-03-05T18:58:51Z
• Authors: Dayang Li; Kunjie Li; Jianquan Li; Dongfang Li; Heng Chen; Sen-Sen Li; Chaoyong Yang; Huimin Zhang; Lu-Jian Chen; Xuejia Hu
• Keywords: Microscale chemistry

Enzymatic nucleic acid reaction is a fundamental tool in molecular biology. However, high-complexity enzymatic DNA reactions and assays are still challenging due to the difficulties integrating scaling up microscale units mixing tools. Here, we present scalable acoustofluidic platform featuring acoustic virtual stirrer (AVS) arrays, serving as stirrers increase efficiency of interfacial reactions. Analogous magnetic stirrers, AVS arrays perturb fluid through oscillating pressure nodes, controllable terms speeds amplitudes via modulation. By optimizing kinetics surface-tethered enzymes AVS, achieve 7.74% improvement stepwise yield synthesis. In addition, enhanced logic gate architecture can complete responses within 2 minutes, achieving average speed enhancement 8.58 times compared non-AVS configuration. With its tunability, ease integration, efficiency, this technology holds promises for applications biology chemistry.