Recruitment of HAT Complexes by Direct Activator Interactions with the ATM-Related Tra1 Subunit

• DOI: 10.1126/science.1060214
• Publication Date: 2002-07-27T09:53:24Z
• Authors: Christine E. Brown; LeAnn Howe; Kyle Sousa; Stephen C. Alley; Michael J. Carrozza; Song Tan; Jerry L. Workman
• Keywords: Histone acetyltransferase; Acetyltransferases; Transcription; Acetyltransferases; SAP30

Promoter-specific recruitment of histone acetyltransferase activity is often critical for transcriptional activation. We present a detailed study the interaction between complexes SAGA and NuA4, transcription activators. demonstrate by affinity chromatography photo–cross-linking label transfer that acidic activators directly interact with Tra1p, shared subunit NuA4. Mutations within COOH-terminus Tra1p disrupted its resulted in gene-specific defects correlated lowered promoter-specific acetylation. These data essential Tra1 protein serves as common target both NuA4 acetyltransferases.