Regulation of Blood and Lymphatic Vascular Separation by Signaling Proteins SLP-76 and Syk

• DOI: 10.1126/science.1079477
• Publication Date: 2003-01-10T01:23:05Z
• Authors: Farhad Abtahian; Anastasia Guerriero; Eric Sebzda; Min-Min Lu; Rong Zhou; Attila Mocsai; Erin E. Myers; Bin Huang; David G. Jackson; Victor A. Ferrari; Victor Tybulewicz; Clifford A. Lowell; John J. Lepore; Gary A. Koretzky; Mark L. Kahn
• Keywords: Lymphatic vessel; Lymphatic Endothelium

Lymphatic vessels develop from specialized endothelial cells in preexisting blood vessels, but the molecular signals that regulate this separation are unknown. Here we identify a failure to separate emerging lymphatic mice lacking hematopoietic signaling protein SLP-76 or Syk. Blood-lymphatic connections lead embryonic hemorrhage and arteriovenous shunting. Expression of slp-76 could not be detected cells, blood-filled lymphatics also arose wild-type reconstituted with SLP-76-deficient bone marrow. These studies reveal pathway required for two major vascular networks mammals.