Collection, Mapping, and Annotation of Over 28,000 cDNA Clones from japonica Rice

• DOI: 10.1126/science.1081288
• Publication Date: 2003-07-17T20:32:21Z
• Authors: Shoshi Kikuchi; Kouji Satoh; Toshifumi Nagata; Nobuyuki Kawagashira; Koji Doi; Naoki Kishimoto; Junshi Yazaki; Masahiro Ishikawa; Hitomi Yamada; Hisako Ooka; Isamu Hotta; Keiichi Kojima; Takahiro Namiki; Eisuke Ohneda; Wataru Yahagi; Kohji Suzuki; Chao Jie Li; Kenji Ohtsuki; Toru Shishiki; Yasuhiro Otomo; Kazuo Murakami; Yoshiharu Iida; Sumio Sugano; Tatsuto Fujimura; Yutaka Suzuki; Yuki Tsunoda; Takashi Kurosaki; Takeko Kodama; Hiromi Masuda; Michie Kobayashi; Quihong Xie; Min Lu; Ryuya Narikawa; Akio Sugiyama; Kouichi Mizuno; Satoko Yokomizo; Junko Niikura; Rieko Ikeda; Junya Ishibiki; Midori Kawamata; Akemi Yoshimura; Junichirou Miura; Takahiro Kusumegi; Mitsuru Oka; Risa Ryu; Mariko Ueda; Kenichi Matsubara; Jun Kawai; Piero Carninci; Jun Adachi; Katsunori Aizawa; Takahiro Arakawa; Shiro Fukuda; Ayako Hara; Wataru Hashidume; Norihito Hayatsu; Koichi Imotani; Yoshiki Ishii; Masayoshi Itoh; Ikuko Kagawa; Shinji Kondo; Hedeaki Konno; Ai Miyazaki; Naoki Osato; Yoshimi Ota; Rintaro Saito; Daisuke Sasaki; Kenjiro Sato; Kazuhiro Shibata; Akira Shinagawa; Toshiyuki Shiraki; Masayasu Yoshino; Yoshihide Hayashi...
• Keywords: Homology; genomic DNA

We collected and completely sequenced 28,469 full-length complementary DNA clones from Oryza sativa L. ssp. japonica cv. Nipponbare. Through homology searches of publicly available sequence data, we assigned tentative protein functions to 21,596 (75.86%). Mapping the cDNA genomic revealed that there are 19,000 20,500 transcription units in rice genome. Protein informatics analysis against InterPro database existence proteins presented but not Arabidopsis. Sixty-four percent our cDNAs homologous Arabidopsis proteins.