Requirement of Mammalian Timeless for Circadian Rhythmicity

• DOI: 10.1126/science.1086593
• Publication Date: 2003-10-16T21:09:09Z
• Authors: Jessica W. Barnes; Shelley A. Tischkau; Jeffrey A. Barnes; Jennifer W. Mitchell; Penny W. Burgoon; Jason R. Hickok; Martha U. Gillette
• Keywords: Timeless; Clockwork; Oscillating gene

Despite a central circadian role in Drosophila for the transcriptional regulator Timeless ( dTim ), relevance of mammalian mTim ) remains equivocal. Conditional knockdown protein expression rat suprachiasmatic nucleus (SCN) disrupted SCN neuronal activity rhythms, and altered levels known core clock elements. Full-length (mTIM-fl) exhibited 24-hour oscillation, where as truncated isoform (mTIM-s) was constitutively expressed. mTIM-fl associated with Period proteins (mPERs) oscillating cells. These data suggest that is required rhythmicity functional homolog on negative-feedback arm molecular clockwork.