A Mule Cloned from Fetal Cells by Nuclear Transfer

• DOI: 10.1126/science.1086743
• Publication Date: 2003-05-29T19:58:24Z
• Authors: Gordon L. Woods; Kenneth L. White; Dirk K. Vanderwall; Guang-Peng Li; Kenneth I. Aston; Thomas D. Bunch; Lora N. Meerdo; Barry J. Pate
• Keywords: clone; Veterinary Medicine; Male; nuclear transfer; Nuclear Transfer Techniques; 0303 health sciences; Cloning, Organism; Dairy Science; fetal cells; Equidae; Fibroblasts; Embryo Transfer; Embryo, Mammalian; mule; Cell Line; Embryonic and Fetal Development; 03 medical and health sciences; Pregnancy; Animal Sciences; Oocytes; Animals; Calcium; Female; Horses

Nuclear transfer was used to produce a cloned mule, a sterile hybrid equine species. Mules result from breeding a male donkey ( Equus asinus , 62 chromosomes) with a female horse ( Equus caballus , 64 chromosomes), producing an animal with 63 chromosomes. Equine cloning has been hampered by a lack