Enrichment of Gene-Coding Sequences in Maize by Genome Filtration

• DOI: 10.1126/science.1090047
• Publication Date: 2003-12-18T22:57:53Z
• Authors: C. A. Whitelaw; W. B. Barbazuk; G. Pertea; A. P. Chan; F. Cheung; Y. Lee; L. Zheng; S. van Heeringen; S. Karamycheva; J. L. Bennetzen; P. SanMiguel; N. Lakey; J. Bedell; Y. Yuan; M. A. Budiman; A. Resnick; S. Van Aken; T. Utterback; S. Riedmuller; M. Williams; T. Feldblyum; K. Schubert; R. Beachy; C. M. Fraser; J. Quackenbush
• Keywords: Genome size

Approximately 80% of the maize genome comprises highly repetitive sequences interspersed with single-copy, gene-rich sequences, and standard sequencing strategies are not readily adaptable to this type genome. Methodologies that enrich for genic might more rapidly generate useful results from complex genomes. Equivalent numbers clones selected by techniques called methylation filtering High C0t selection were sequenced approximately 200,000 reads (approximately 132 megabases), which assembled into contigs. Combination two resulted in a sixfold reduction effective size fourfold increase gene identification rate comparison nonenriched library.