Genetic Dissection of Complex Traits with Chromosome Substitution Strains of Mice

• DOI: 10.1126/science.1093139
• Publication Date: 2004-03-23T01:23:55Z
• Authors: Jonathan B. Singer; Annie E. Hill; Lindsay C. Burrage; Keith R. Olszens; Junghan Song; Monica Justice; William E. O'Brien; David V. Conti; John S. Witte; Eric S. Lander; Joseph H. Nadeau
• Keywords: Inbred strain; Strain (injury)

Chromosome substitution strains (CSSs) have been proposed as a simple and powerful way to identify quantitative trait loci (QTLs) affecting developmental, physiological, behavioral processes. Here, we report the construction of complete CSS panel for vertebrate species. The consists 22 mouse strains, each which carries single chromosome substituted from donor strain (A/J) onto common host background (C57BL/6J). A survey 53 traits revealed evidence 150 QTLs serum levels sterols amino acids, diet-induced obesity, anxiety. These results demonstrate that CSSs greatly facilitate detection identification genes control wide diversity naturally occurring phenotypic variation in A/J C57BL/6J inbred strains.