Vaccinia Virus-Induced Cell Motility Requires F11L-Mediated Inhibition of RhoA Signaling

0301 basic medicine 0303 health sciences Genes, Viral Pyridines Recombinant Fusion Proteins Intracellular Signaling Peptides and Proteins Protein Serine-Threonine Kinases Phosphoproteins Amides Cell Line Protein Structure, Tertiary 3. Good health Cytoskeletal Proteins 03 medical and health sciences Cell Movement Morphogenesis Animals Humans RNA Interference Enzyme Inhibitors RNA, Small Interfering HeLa Cells Protein Binding Signal Transduction
DOI: 10.1126/science.1122411 Publication Date: 2006-01-19T22:08:37Z
ABSTRACT
RhoA signaling plays a critical role in many cellular processes, including cell migration. Here we show that the vaccinia F11L protein interacts directly with RhoA, inhibiting its by blocking interaction downstream effectors Rho-associated kinase (ROCK) and mDia. RNA interference-mediated depletion of during infection resulted an absence vaccinia-induced motility inhibition viral morphogenesis. Disruption binding site F11L, which resembles ROCK, led to identical phenotype. Thus, is required for both morphogenesis virus-induced motility.
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