Vaccinia Virus-Induced Cell Motility Requires F11L-Mediated Inhibition of RhoA Signaling

• DOI: 10.1126/science.1122411
• Publication Date: 2006-01-19T22:08:37Z
• Authors: Ferran Valderrama; João V. Cordeiro; Sibylle Schleich; Friedrich Frischk...; Michael Way
• Keywords: 0301 basic medicine; 0303 health sciences; Genes, Viral; Pyridines; Recombinant Fusion Proteins; Intracellular Signaling Peptides and Proteins; Protein Serine-Threonine Kinases; Phosphoproteins; Amides; Cell Line; Protein Structure, Tertiary; 3. Good health; Cytoskeletal Proteins; 03 medical and health sciences; Cell Movement; Morphogenesis; Animals; Humans; RNA Interference; Enzyme Inhibitors; RNA, Small Interfering; HeLa Cells; Protein Binding; Signal Transduction

RhoA signaling plays a critical role in many cellular processes, including cell migration. Here we show that the vaccinia F11L protein interacts directly with RhoA, inhibiting its by blocking interaction downstream effectors Rho-associated kinase (ROCK) and mDia. RNA interference-mediated depletion of during infection resulted an absence vaccinia-induced motility inhibition viral morphogenesis. Disruption binding site F11L, which resembles ROCK, led to identical phenotype. Thus, is required for both morphogenesis virus-induced motility.