Signal Recognition Particle Receptor Exposes the Ribosomal Translocon Binding Site

• DOI: 10.1126/science.1124864
• Publication Date: 2006-05-04T21:04:25Z
• Authors: Mario Halic; Marco Gartmann; Oliver Schlenker; Thorsten Mielke; Martin R. Pool; Irmgard Sinning; Roland Beckmann
• Keywords: Signal recognition particle; A-site

Signal sequences of secretory and membrane proteins are recognized by the signal recognition particle (SRP) as they emerge from ribosome. This results in their targeting to docking with SRP receptor, which facilitates transfer ribosome translocon. Here, we present 8 angstrom cryo-electron microscopy structure a "docking complex" consisting SRP-bound 80S receptor. Interaction receptor both rearranged S domain such that ribosomal binding site for translocon, L23e/L35 site, became exposed, whereas Alu domain-mediated elongation arrest persisted.