NADP Regulates the Yeast GAL Induction System

• DOI: 10.1126/science.1151903
• Publication Date: 2008-02-21T21:54:09Z
• Authors: P. Rajesh Kumar; Yao Yu; Rolf Sternglanz; Stephen Albert Jo...; Leemor Joshua-Tor
• Keywords: Transcription

Transcriptional regulation of the galactose-metabolizing genes in Saccharomyces cerevisiae depends on three core proteins: Gal4p, transcriptional activator that binds to upstream activating DNA sequences (UAS(GAL)); Gal80p, a repressor carboxyl terminus Gal4p and inhibits transcription; Gal3p, cytoplasmic transducer that, upon binding galactose adenosine 5'-triphosphate, relieves Gal80p repression. The current model induction relies Gal3p sequestering cytoplasm. However, rapid this system implies there is missing factor. Our structure complex with peptide from carboxyl-terminal activation domain reveals existence dinucleotide mediates interaction between two. Biochemical vivo experiments suggests nicotinamide adenine phosphate (NADP) plays key role initial event.