Identification of Host Proteins Required for HIV Infection Through a Functional Genomic Screen

• DOI: 10.1126/science.1152725
• Publication Date: 2008-01-11T01:59:46Z
• Authors: Abraham L. Brass; Derek M. Dykxhoorn; Yair Benita; Nan Yan; Alan Engelman; Ramnik J. Xavier; Judy Lieberman; Stephen J. Elledge
• Keywords: Viral life cycle

HIV-1 exploits multiple host proteins during infection. We performed a large-scale small interfering RNA screen to identify factors required by and identified more than 250 HIV-dependency (HDFs). These participate in broad array of cellular functions implicate new pathways the viral life cycle. Further analysis revealed previously unknown roles for retrograde Golgi transport (Rab6 Vps53) entry, karyopherin (TNPO3) integration, Mediator complex (Med28) transcription. Transcriptional that HDF genes were enriched high expression immune cells, suggesting viruses evolve cells optimally perform their This effort illustrates power with which interference forward genetics can be used expose dependencies human pathogens such as HIV, so doing potential targets therapy.