Generation of Mouse Induced Pluripotent Stem Cells Without Viral Vectors
Pluripotent Stem Cells
0301 basic medicine
DNA, Complementary
Chimera
SOXB1 Transcription Factors
Genetic Vectors
Genes, myc
Kruppel-Like Transcription Factors
Teratoma
Fibroblasts
Cellular Reprogramming
Embryo, Mammalian
Transfection
3. Good health
Kruppel-Like Factor 4
Mice
03 medical and health sciences
Retroviridae
Animals
Octamer Transcription Factor-3
Embryonic Stem Cells
Plasmids
DOI:
10.1126/science.1164270
Publication Date:
2008-10-10T01:39:41Z
AUTHORS (5)
ABSTRACT
Induced pluripotent stem (iPS) cells have been generated from mouse and human somatic cells by introducing Oct3/4 and Sox2 with either Klf4 and c-Myc or Nanog and Lin28 using retroviruses or lentiviruses. Patient-specific iPS cells could be useful in drug discovery and regenerative medicine. However, viral integration into the host genome increases the risk of tumorigenicity. Here, we report the generation of mouse iPS cells without viral vectors. Repeated transfection of two expression plasmids, one containing the complementary DNAs (cDNAs) of Oct3/4, Sox2, and Klf4 and the other containing the c-Myc cDNA, into mouse embryonic fibroblasts resulted in iPS cells without evidence of plasmid integration, which produced teratomas when transplanted into mice and contributed to adult chimeras. The production of virus-free iPS cells, albeit from embryonic fibroblasts, addresses a critical safety concern for potential use of iPS cells in regenerative medicine.
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