Sequence of the Cloned Gene for the Constant Region of Murine γ2b Immunoglobulin Heavy Chain

• DOI: 10.1126/science.117549
• Publication Date: 2006-10-05T16:39:42Z
• Authors: Philip W. Tucker; Kenneth B. Marcu; Nanette Newell; Julia Richards; Frederick R. Blat...
• Keywords: 0301 basic medicine; Mice, Inbred BALB C; Base Sequence; Transcription, Genetic; Immunoglobulin gamma-Chains; DNA, Recombinant; Immunoglobulins; Nucleic Acid Precursors; Biological Evolution; 3. Good health; Mice; 03 medical and health sciences; Genes; Liver; Animals; RNA, Messenger; Immunoglobulin Constant Regions; Immunoglobulin Heavy Chains

The complete nucleotide sequence of the γ2b constant region gene cloned from BALB/c liver DNA is reported. The sequence of approximately 1870 base pairs includes the 5′ flanking, 3′ untranslated, and 3′ flanking regions and three introns. The Cγ2b coding region is divided by these introns into four segments corresponding to the homology domains and hinge region of the protein. The introns separating the hinge from the C H 2 domain and the C H 2 from the C H 3 domain are small (106 and 119 base pairs). A larger intervening sequence of 314 base pairs separates the C H 1 and hinge regions. The stretch of DNA comprising this large intron plus the hinge shows a strong homology with the other C H domains.