Poly(ADP-ribose)–Dependent Regulation of DNA Repair by the Chromatin Remodeling Enzyme ALC1

• DOI: 10.1126/science.1177321
• Publication Date: 2009-08-07T01:55:26Z
• Authors: Dragana Ahel; Zuzana Hořejší; Nicola Wiechens; Sophie E. Polo; Elisa Garcia-Wilson; Ivan Ahel; Helen Flynn; Mark Skehel; Stephen C. West; Stephen P. Jackson; Tom Owen-Hughes; Simon J. Boulton
• Keywords: PARP1

Posttranslational modifications play key roles in regulating chromatin plasticity. Although various chromatin-remodeling enzymes have been described that respond to specific histone modifications, little is known about the role of poly[adenosine 5'-diphosphate (ADP)-ribose] remodeling. Here, we identify a enzyme, ALC1 (Amplified Liver Cancer 1, also as CHD1L), interacts with poly(ADP-ribose) and catalyzes PARP1-stimulated nucleosome sliding. Our results define DNA damage-response protein whose this process sustained by its association repair factors rapid poly(ADP-ribose)-dependent recruitment damage sites. Furthermore, show depletion or overexpression sensitivity DNA-damaging agents. Collectively, these provide new insights into mechanisms which regulates repair.