Systematic Analysis of Human Protein Complexes Identifies Chromosome Segregation Proteins
Centrosome
Chromosomes, Artificial, Bacterial
Green Fluorescent Proteins
Mitosis
Ubiquitin-Protein Ligase Complexes
Genomics
Spindle Apparatus
Anaphase-Promoting Complex-Cyclosome
Open Reading Frames
Protein Subunits
Tubulin
Chromosome Segregation
Multiprotein Complexes
Databases, Genetic
Protein Interaction Mapping
Humans
RNA Interference
HeLa Cells
Protein Binding
DOI:
10.1126/science.1181348
Publication Date:
2010-04-02T02:27:57Z
AUTHORS (30)
ABSTRACT
Chromosome segregation and cell division are essential, highly ordered processes that depend on numerous protein complexes. Results from recent RNA interference screens indicate the identity composition of these complexes is incompletely understood. Using gene tagging bacterial artificial chromosomes, localization, tandem-affinity purification-mass spectrometry, MitoCheck consortium has analyzed about 100 human complexes, many which had not or only been characterized. This work led to discovery previously unknown, evolutionarily conserved subunits anaphase-promoting complex gamma-tubulin ring complex--large essential for spindle assembly chromosome segregation. The approaches we describe here generally applicable high-throughput follow-up analyses phenotypic in mammalian cells.
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CITATIONS (444)
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