Systematic Analysis of Human Protein Complexes Identifies Chromosome Segregation Proteins

• DOI: 10.1126/science.1181348
• Publication Date: 2010-04-02T02:27:57Z
• Authors: James R. A. Hutchins; Yusuke Toyoda; Björn Hegemann; Ina Poser; Jean-Karim Hériché; Martina M. Sykora; Martina Augsburg; Otto Hudecz; Bettina A. Buschhorn; Jutta Bulkescher; Christian Conrad; David Comartin; Alexander Schleiffer; Mihail Sarov; Andrei Pozniakovsky; Mikolaj Michal Sl...; Siegfried Schlois...; Ines Steinmacher; Marit Leuschner; Andrea Ssykor; Steffen Lawo; Laurence Pelletier; Holger Stark; Kim Nasmyth; Jan Ellenberg; Richard Durbin; Frank Buchholz; Karl Mechtler; Anthony A. Hyman; Jan-Michael Peters
• Keywords: Centrosome; Chromosomes, Artificial, Bacterial; Green Fluorescent Proteins; Mitosis; Ubiquitin-Protein Ligase Complexes; Genomics; Spindle Apparatus; Anaphase-Promoting Complex-Cyclosome; Open Reading Frames; Protein Subunits; Tubulin; Chromosome Segregation; Multiprotein Complexes; Databases, Genetic; Protein Interaction Mapping; Humans; RNA Interference; HeLa Cells; Protein Binding

Division Machinery Tagged

An international consortium of labs has been testing the feasibility of large-scale screening for insights into the function of mammalian proteins by expressing a tagged version of proteins from bacterial artificial chromosomes harbored in mammalian cells. Depending on the tag used,

Hutchins et al.

(p. 593 , published online 1 April) were able to monitor localization of tagged proteins by microscopy or to isolate interacting proteins and subsequently identify the binding partners by mass spectrometry. Applying the technology to proteins implicated in control of cell division revealed about 100 protein machines required for mitosis.