Systematic Analysis of Human Protein Complexes Identifies Chromosome Segregation Proteins

Centrosome Chromosomes, Artificial, Bacterial Green Fluorescent Proteins Mitosis Ubiquitin-Protein Ligase Complexes Genomics Spindle Apparatus Anaphase-Promoting Complex-Cyclosome Open Reading Frames Protein Subunits Tubulin Chromosome Segregation Multiprotein Complexes Databases, Genetic Protein Interaction Mapping Humans RNA Interference HeLa Cells Protein Binding
DOI: 10.1126/science.1181348 Publication Date: 2010-04-02T02:27:57Z
ABSTRACT
Division Machinery Tagged An international consortium of labs has been testing the feasibility of large-scale screening for insights into the function of mammalian proteins by expressing a tagged version of proteins from bacterial artificial chromosomes harbored in mammalian cells. Depending on the tag used, Hutchins et al. (p. 593 , published online 1 April) were able to monitor localization of tagged proteins by microscopy or to isolate interacting proteins and subsequently identify the binding partners by mass spectrometry. Applying the technology to proteins implicated in control of cell division revealed about 100 protein machines required for mitosis.
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