Systematic Analysis of Human Protein Complexes Identifies Chromosome Segregation Proteins
Centrosome
Chromosomes, Artificial, Bacterial
Green Fluorescent Proteins
Mitosis
Ubiquitin-Protein Ligase Complexes
Genomics
Spindle Apparatus
Anaphase-Promoting Complex-Cyclosome
Open Reading Frames
Protein Subunits
Tubulin
Chromosome Segregation
Multiprotein Complexes
Databases, Genetic
Protein Interaction Mapping
Humans
RNA Interference
HeLa Cells
Protein Binding
DOI:
10.1126/science.1181348
Publication Date:
2010-04-02T02:27:57Z
AUTHORS (30)
ABSTRACT
Division Machinery Tagged
An international consortium of labs has been testing the feasibility of large-scale screening for insights into the function of mammalian proteins by expressing a tagged version of proteins from bacterial artificial chromosomes harbored in mammalian cells. Depending on the tag used,
Hutchins
et al.
(p.
593
, published online 1 April) were able to monitor localization of tagged proteins by microscopy or to isolate interacting proteins and subsequently identify the binding partners by mass spectrometry. Applying the technology to proteins implicated in control of cell division revealed about 100 protein machines required for mitosis.
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