Systematic Analysis of Human Protein Complexes Identifies Chromosome Segregation Proteins

Centrosome Chromosomes, Artificial, Bacterial Green Fluorescent Proteins Mitosis Ubiquitin-Protein Ligase Complexes Genomics Spindle Apparatus Anaphase-Promoting Complex-Cyclosome Open Reading Frames Protein Subunits Tubulin Chromosome Segregation Multiprotein Complexes Databases, Genetic Protein Interaction Mapping Humans RNA Interference HeLa Cells Protein Binding
DOI: 10.1126/science.1181348 Publication Date: 2010-04-02T02:27:57Z
ABSTRACT
Chromosome segregation and cell division are essential, highly ordered processes that depend on numerous protein complexes. Results from recent RNA interference screens indicate the identity composition of these complexes is incompletely understood. Using gene tagging bacterial artificial chromosomes, localization, tandem-affinity purification-mass spectrometry, MitoCheck consortium has analyzed about 100 human complexes, many which had not or only been characterized. This work led to discovery previously unknown, evolutionarily conserved subunits anaphase-promoting complex gamma-tubulin ring complex--large essential for spindle assembly chromosome segregation. The approaches we describe here generally applicable high-throughput follow-up analyses phenotypic in mammalian cells.
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