Cbln1 Is a Ligand for an Orphan Glutamate Receptor δ2, a Bidirectional Synapse Organizer

• DOI: 10.1126/science.1185152
• Publication Date: 2010-04-15T18:30:21Z
• Authors: Keiko Matsuda; Eriko Miura; Taisuke Miyazaki; Wataru Kakegawa; Kyoichi Emi; Sakae Narumi; Yugo Fukazawa; Aya Ito-Ishida; Tetsuro Kondo; Ryuichi Shigemoto; Masahiko Watanabe; Michisuke Yuzaki
• Keywords: Deep cerebellar nuclei; Orphan receptor

Cbln1, secreted from cerebellar granule cells, and the orphan glutamate receptor delta2 (GluD2), expressed by Purkinje are essential for synapse integrity between these neurons in adult mice. Nevertheless, no endogenous binding partners molecules have been identified. We found that Cbln1 binds directly to N-terminal domain of GluD2. GluD2 expression postsynaptic combined with exogenously applied was necessary sufficient induce new synapses vitro cerebellum vivo. Further, beads coated recombinant induced presynaptic differentiation indirectly caused clustering via These results indicate Cbln1-GluD2 complex is a unique organizer acts bidirectionally on both pre- components.