GFAJ-1 Is an Arsenate-Resistant, Phosphate-Dependent Organism

• DOI: 10.1126/science.1218455
• Publication Date: 2012-07-09T00:13:49Z
• Authors: Tobias J. Erb; Patrick Kiefer; Bodo Hattendorf; Detlef Günther; Julia A. Vorholt
• Keywords: DNA, Bacterial; 0303 health sciences; Nucleotides; Phosphorus; Mass Spectrometry; 6. Clean water; Arsenic; Culture Media; Phosphates; Halomonadaceae; RNA, Bacterial; 03 medical and health sciences; Drug Resistance, Bacterial; Metabolome; Arsenates; Hexosephosphates; Phosphorylation; Glycolysis; Hexoses

Resisting Arsenic

The discovery of a bacterium living in the extreme conditions of Mono Lake, California, created a major controversy because it was claimed to be able to grow solely on arsenic and could substitute arsenate for phosphate in its key macromolecules, including DNA. Working with the same Halomonas spp. bacterium, known as GFAJ-1, and ultrapure reagents,

Erb et al.

(p. 467 ) found that the bacterium needed a low level of phosphate (1.6 µM) to grow at all. Rather than significant specific arsenic incorporation, when the organism was grown in 40 mM arsenic, its nucleic acids acquired a trace of arsenic. Similarly,

Reaves et al.

(p. 470 ) found that GFAJ-1 could not grow in the absence of phosphate and, moreover, that its growth was not stimulated by the addition of arsenate, although a trace amount of arsenic was also detected in DNA. Thus, GFAJ-1 shows no particular facility to substitute arsenic for phosphate, when phosphate is limiting, but it can tolerate high concentrations of the poison while efficiently scavenging phosphate.