Metabolite Profiling Identifies a Key Role for Glycine in Rapid Cancer Cell Proliferation

• DOI: 10.1126/science.1218595
• Publication Date: 2012-05-24T18:06:31Z
• Authors: Mohit Jain; Roland Nilsson; Sonia Sharma; Nikhil Madhusudhan; Toshimori Kitami; Amanda L. Souza; Ran Kafri; Marc W. Kirschner; Clary B. Clish; Vamsi K. Mootha
• Keywords: Gene Expression Profiling; Cell Cycle; Glycine; Gene Expression; Breast Neoplasms; Cell Line; Culture Media; Mitochondria; 3. Good health; Cell Transformation, Neoplastic; Purines; Tandem Mass Spectrometry; Cell Line, Tumor; Neoplasms; Metabolome; Humans; Metabolic Networks and Pathways; Cell Proliferation; Chromatography, Liquid

More Glycine, PleaseTo better characterize metabolic properties of cancer cells,Jainet al.(p.1040; see the Perspective byTomita and Kami) measured systematically the concentrations of hundreds of metabolites in cell culture medium in which 60 different cancer cell lines were growing. The fastest growing cancer cells tended to consume glycine, whereas more slowly growing cells excreted some glycine. The rapidly growing cancer cells appeared to need glycine for synthesis of purine nucleotides required for continued synthesis of DNA. Interfering with glycine metabolism slowed growth of the rapidly proliferating cancer cells. Thus, an increased dependence on glycine by rapidly growing cancer cells could potentially provide a target for therapeutic intervention.