Massively Parallel Single-Cell RNA-Seq for Marker-Free Decomposition of Tissues into Cell Types

• DOI: 10.1126/science.1247651
• Publication Date: 2014-02-14T17:04:58Z
• Authors: Diego Adhemar Jaitin; Ephraim Kenigsberg; Hadas Keren-Shaul; Naama Elefant; Franziska Paul; Irina Zaretsky; Alexander Mildner; Nadav Cohen; Steffen Jung; Amos Tanay; Ido Amit
• Keywords: 0303 health sciences; Transcription, Genetic; Sequence Analysis, RNA; Dendritic Cells; Hematopoiesis; Mice, Inbred C57BL; 03 medical and health sciences; Animals; Female; RNA, Messenger; Single-Cell Analysis; Biomarkers; Spleen

Sequencing of RNA from thousands of individual immune cells allows unbiased identification of cellular subtypes.