A single-stranded architecture for cotranscriptional folding of RNA nanostructures

• DOI: 10.1126/science.1253920
• Publication Date: 2014-08-14T18:25:21Z
• Authors: Cody Geary; Paul W. K. Rothemund; Ebbe S. Andersen
• Keywords: DNA origami; Folding (DSP implementation); DNA nanotechnology; Nucleic acid structure

Artificial DNA and RNA structures have been used as scaffolds for a variety of nanoscale devices. In comparison to structures, limited in size, but they also advantages: can fold during transcription thus be genetically encoded expressed cells. We introduce an architecture designing artificial that from single strand, which arrays antiparallel helices are precisely organized by tertiary motifs new type crossover pattern. constructed tiles assemble into hexagonal lattices demonstrated made annealing and/or cotranscriptional folding. Tiles scaled up 660 nucleotides length, reaching size comparable large natural ribozymes.