Density Gradient Centrifugation of a Murine Leukemia Virus

• DOI: 10.1126/science.144.3622.1144
• Publication Date: 2006-10-05T09:47:29Z
• Authors: T. E. O'Connor; F. J. Rauscher; R. F. Zeigel
• Keywords: Density gradient; Rubidium; Tartrate; Differential centrifugation; Caesium

The Rauscher leukemia virus separated as a single band upon density gradient centrifugation in cesium chloride, rubidium sucrose, potassium citrate, or tartrate. Prolonged exposure to concentrated citrate tartrate solutions caused lysis of the virus; resulting products, having different densities, on gradients.