Lambda Int Protein Bridges Between Higher Order Complexes at Two Distant Chromosomal Loci att L and att R

• DOI: 10.1126/science.1533056
• Publication Date: 2006-10-05T23:03:06Z
• Authors: Sunghoon Kim; Arthur Landy
• Keywords: Synapsis

The excisive recombination reaction of bacteriophage lambda involves a specific and efficient juxtaposition two distant higher order protein-DNA complexes on the chromosome Escherichia coli . These complexes, which mediate synapsis strand exchange, consist DNA sequences, att L R, bivalent binding protein Int, sequence-specific bending proteins, IHF, Xis, Fis. protein-protein interactions within, between, these were studied by various biochemical techniques patterns synergism among pairs mutants with marginally impaired function analyzed. proteins facilitated long-range tethering high- low-affinity sites Int protein, map is proposed for resulting bridges. structural motifs provide basis postulating mechanisms site-specific may also be relevant to other pathways in chromosomal become associated.